RBS

Part:BBa_K4159001:Design

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-14)


RBS (Olins and Rangwala, 1989)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This RBS sequence has been used before [2] in ribosome display. No changes to the sequence needed in comparison to the source [1].


Source

[1] Peter O. Olins, Catherine S. Devine, Shaukat H. Rangwala, Kamilla S. Kavka, The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli, Gene,Volume 73, Issue 1, 1988, Pages 227-235, ISSN 0378-1119, https://doi.org/10.1016/0378-1119(88)90329-0. [2] Chen X, Gentili M, Hacohen N, Regev A. A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies. Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z. PMID: 34535642; PMCID: PMC8448731. (https://www.sciencedirect.com/science/article/pii/0378111988903290)

References